Jasco J-815 Spectropolarimeter

J-815 circular dichroism spectrometer

Circular Dichroism (CD) spectroscopy measures differences in the absorption of left-handed polarized light versus right-handed polarized light that arise due to structural asymmetry. The absence of regular structure results in zero CD intensity, while an ordered structure results in a spectrum which can contain both positive and negative signals.

Applications

• Protein folding studies
• Protein conformational studies
• DNA/RNA interactions
• Enzyme kinetics
• Purity testing of optically active substances
• Quantitative analysis of pharmaceuticals
• Natural organic chemistry
• Biochemistry and macromolecules
• Rapid scanning (time resolved) experiments

Condition:

This instrument is in good working order and was removed from working environment. Unit is in excellent condition.

Warranty:

60-Da

Testing we performed:

Passes all initializing self and basic functionality tests. Display controller keypad panel and lamp operate properly.

Specifications:

Light source: 150W air-cooled Xe lamp or 450W water-cooled Xe lamp (factory option)

Measurement wavelength range: 163 to 900 nm (standard detector); 163 to 1100 nm (optional detector)

Wavelength accuracy: ±0.2 nm (at 163 to 180 nm); ±0.1 nm (at 180 to 250 nm); ±0.3 nm (at 250 to 500 nm); ±0.8 nm (at 500 to 800 nm), ±2.0 nm (at 800 to 1100 nm)

Wavelength repeatability: ±0.05 nm (at 163 to 250 nm); ±0.1 nm (at 250 to 500 nm); ±0.2 nm (at 500 to 1100 nm)

Spectral bandwidth: 0.01 to 15 nm

Slit width: 1 to 3000 µm

Digital Integration Time (D.I.T.): 0.5 msec to 32 sec

Acquisition modes: Wavelength scan (3 modes), Time scan (slow and fast kinetics), Temperature scan

Scanning speeds: 1 to 10000 nm/min (continuous scan)

Data interval: 0.025 to 10 nm (continuous scan); 0.1 to 100 nm (step scan); 0.5 msec to 60 min (time course)

CD full scale: ±10, 200, 2000 mdeg

CD resolution: 0.0005 mdeg (at ±10 mdeg full scale); 0.01 mdeg ( at ±200 mdeg full scale); 0.1 mdeg ( at ±2000 mdeg full scale)

Stray light: Less than 0.0003% (200 nm)

Dimensions: 1115 (W) × 576 (D) × 410 (H) mm

Weight: 87 kg